منابع مشابه
Puzzling Peptides from a Phage Display Library
The commercial availability of random peptide libraries displayed on the M13 phage is increasing their use forstudies on epitope identification, enzyme inhibitors, receptor ligands, etc. In this study two experimentswhere planned for selection of peptides. First with sheep antibodies, the positive selector was IgG, preparedon Protein G column from a pool of 11 sheeps immunized...
متن کاملPhage Display.
In crossing a heath, suppose...I had found a watch upon the ground, and it should be inquired how the watch happened to be in that place....[T]he inference, we think, is inevitable, that the watch must have had a maker: that there must have existed...an artificer or artificers, who formed it for the purpose which we find it actually to answer; who comprehended its construction and designed its ...
متن کاملscreening phage display library for isolation of human colon cancer cell-binding peptide ligands
objective: recently, phage display libraries have received enormous attention for identification and isolation of pharmaceutical molecules with diagnostic and therapeutic properties. peptide libraries are known as one of the most important and widely used types of phage display libraries. in the current study, we aimed to screen the ph.d.tm-7 phage display peptide library through biopanning for...
متن کاملIdentification of gliadin-binding peptides by phage display
BACKGROUND Coeliac disease (CD) is a common and complex disorder of the small intestine caused by intolerance to wheat gluten and related edible cereals like barley and rye. Peptides originating from incomplete gliadin digestion activate the lamina propria infiltrating T cells to release proinflammatory cytokines, which in turn cause profound tissue remodelling of the small intestinal wall. The...
متن کاملEliminating helper phage from phage display
Phage display technology involves the display of proteins or peptides, as coat protein fusions, on the surface of a phage or phagemid particles. Using standard technology, helper phage are essential for the replication and assembly of phagemid particles, during library production and biopanning. We have eliminated the need to add helper phage by using 'bacterial packaging cell lines' that provi...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2010
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113537